| 摘要 |
METHOD OF PRODUCING A PRODUCT PROTEIN, WHEREIN THE PROTEIN IS EXPRESSED FROM A LYMPHOID CELL IN CELL CULTURE AT LEAST DURING A CERTAIN SPAN OF TIME DURING CELL CULTURE, COMPRISING THE STEPS (A) PREPARING A PROTEIN-FREE CELL CULTURE GROWTH MEDIUM, (B) AND FURTHER ADDING ACETIC ACID OR AN ACETATE SALT OR AN ACETYL ESTER TO A FINAL CONCENTRATION OF FROM 3 TO 20 MM, SAID ADDITION BEING CARRIED OUT EITHER DIRECTLY TO THE MEDIUM PRIOR TO OR AT STARTING THE CELL CULTURE OR FEEDING IT TO THE MEDIUM DURING CELL CULTURE, (C) FURTHER CULTURING, PREFERABLY GROWING, SAID CELL IN SAID MEDIUM WITH CONCOMITTANT EXPRESSION OF PRODUCT PROTEIN, (D) AND FINALLY HARVESTING SAID PROTEIN FROM THE CELL CULTURE.
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