Method of generating single VL domain antibodies in transgenic animals

摘要

The present invention describes a single variable domain comprising a human VL domain segment, a DH domain segment, and a human JL domain segment. Provided is also a polynucleotide encoding a respective single variable domain. Further provided are a non-human mammalian cell with a genome that encodes the single variable domain and a hybridoma cell derived from such a non-human mammal.