METHOD OF PRODUCING BIOLOGICAL MATERIAL FOR ENZYME-LINKED IMMUNOASSAY

摘要

FIELD: medicine. ^ SUBSTANCE: invention relates to the medicine, in particular to immunology. Aspirate sampling from uterine cavity is taken for method of preparation of biological material for enzyme-linked immunoassay with plastic catheter. Phosphate buffer (pH 7.4) and nonpolar detergent in the ratio 100:1 are added to aspirate, and keep for 1 hour at the temperature 20-30C, centrifugate 3-5 min. at the rate 3000 r/min. Supernatant fluid is taken for subsequent analysis. ^ EFFECT: it can be applied when evaluating immune system state of women with proliferative diseases of endometrium by testing cytokine content in aspirates from uterine cavity. ^ 3 tbl, 3 ex