发明名称 |
Method for selectively detecting subsets of nucleic acid molecules |
摘要 |
Method for selectively detecting nucleic acid molecules comprising structural aberrations that are capable of being convented into nicks comprising generating linear nucleic acids from a selected nucleic acid substrate population; denaturing and re-annealing the linear nucleic acids to form nucleic acid duplexes; masking the nucleic acid duplex termini and internal structural aberrations with a masking component; modifying the masked nucleic acids by introducing nicks therein using at least an enzyme possessing endonuclease activity: labelling the modified nucleic acids with labelled nucleotides via nucleic acid nick translation with at least an enzyme displaying a nucleic acid polymerase activity; and selecting and identifying the labelled nucleic acid.
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申请公布号 |
US2009215034(A1) |
申请公布日期 |
2009.08.27 |
申请号 |
US20050578001 |
申请日期 |
2005.04.14 |
申请人 |
ORYZON CENOMICS, S. A, |
发明人 |
MAES TAMARA;HAMPSON RICHARD;AMENGUAL MARIA DEL MAR BENITO |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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