METHOD FOR IN VITRO DIAGNOSIS OF PVL-PRODUCING STAPHYLOCOCCUS AUREUS

摘要

#CMT# #/CMT# In vitro diagnosis of Staphylococcus aureus producing Panton Valentine Leukocidin (PVL) using a biological sample from an individual liable to be colonized by or infected with Staphylococcus aureus, comprises detection of PVL by a routine immunological test, where the biological sample is pretreated in order to denature the PVL. #CMT#USE : #/CMT# The method is useful for in vitro diagnosis of staphylococcus aureus. #CMT#ADVANTAGE : #/CMT# The method is simple. The method is direct method because they assert the gene, which is functional and/or expressed. The method does not require special equipments, hence cost effective. The method is easy to implement. The method is devoid of polymerizes chain reaction contamination problem. #CMT#BIOLOGY : #/CMT# Preferred Method: The routine immunological test is a sandwich method. The immunological test implements at least a monoclonal anti-PVL antibody. The pretreatment comprises heating at a temperature of 60-100[deg] C for at least 10 minutes. The method further comprises determining whether the Staphylococcus aureus in the biological sample, which are methicillin resistant bacteria (Methicillin-resistant Staphylococcus aureus) or methicillin-sensitive bacteria (Methicillin-sensitive Staphylococcus aureus).