| 摘要 |
The present invention provides a specimen preparation method for mass spectrometry based on Matrix Assisted Laser Desorption Ionization (MALDI), wherein the method enables to form microcrystals (cocrystals) between matrices and biological molecules (protein and the like) on a biological tissue to generate ions thereof highly efficiently and to perform highly sensitive measurement. Microcrystals are formed on the specimen containing biological molecules, i.e. objects of the measurement, by spraying matrix solution beforehand. Furthermore, dispensation of matrix solution on the microcrystals allows crystals to grow by making preformed micro-matrix crystals as crystal nuclei. Therefore, much finer and more homogeneous crystals (cocrystals) are prepared to enable to perform highly sensitive mass spectrometry based on MALDI. The present invention is a specimen preparation method for mass spectrometry based on matrix assisted laser desorption ionization, wherein the method comprises steps forming microcrystals of matrices on the specimen by spraying matrix solution on the specimen and allowing the microcrystals to grow further by dispensing matrix solutions to the specimen.
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