发明名称 |
Absolute quantification of proteins and modified forms thereof by multistage mass spectrometry |
摘要 |
The invention provides reagents, kits and methods for detecting and/or quantifying proteins in complex mixtures, such as a cell lysate. The methods can be used in high throughput assays to profile cellular proteomes. In one aspect, the invention provides a peptide internal standard labeled with a stable isotope and corresponding in amino acid sequence to the amino acid sequence of a subsequence of a target polypeptide. In another aspect, the peptide internal standard is labeled at a modified amino acid residue and is used to determine the presence of, and/or quantitate the amount of a particular modified form of a protein.
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申请公布号 |
US7501286(B2) |
申请公布日期 |
2009.03.10 |
申请号 |
US20040781047 |
申请日期 |
2004.02.17 |
申请人 |
PRESIDENT AND FELLOWS OF HARVARD COLLEGE |
发明人 |
GYGI STEVEN P.;GERBER SCOTT ANTHONY |
分类号 |
G01N24/00;C07K14/00;G01N33/53;G01N33/68 |
主分类号 |
G01N24/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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