METHOD FOR ANALYZING/IDENTIFYING T-CELL ANTIGEN RECEPTOR GENE AT ONE CELL LEVEL

摘要

PROBLEM TO BE SOLVED: To provide a technique for efficiently analyzing/identifying one human T-cell antigen receptor (TCR) gene, and to provide a transformed human T-cell or the like manifesting the human TCR originated from the one T-cell.

SOLUTION: A CTL cell considered to produce the TCR to gp100209-217peptide amplified in a melanoma patient administered with a dendritic cell vaccine is doubly stained by an FITC-labeled anti-CD8 antibody and a PE-labeled gp100-HLA-A2 tetramer, and the doubly stained CTL cell is fractionated by one cell by using a micromesh device or the like. The whole RNA is extracted from the separated one human CTL cell, and the antigen receptor gene of the one human T-cell is analyzed and identified. A naive T-cell is transformed by incorporating the identified TCR gene.

COPYRIGHT: (C)2009,JPO&INPIT