摘要 |
<p>PURPOSE:To improve the raising rate for the title haploid for acquiring a fixed variety in a short time by cutting the inflorescences of a Cruciferae vegetable after bolting, putting said inflorescences to a specific treatment in a water- cultured state and by culture of the anther or pollen derived from said inflorescences. CONSTITUTION:The inflorescences of a Cruciferae vegetable such as (Chinese) cabbage after bolting are cut and put to low-temperature treatment at 1-10 deg.C for 1-3 days in such a state as to be inserted into water containing nutrients such as nitrogen and phosphoric acid. The anther or pollen derived from the resulting inflorescences is then put to culture to produce embryo-like form, thus raising the objective haploid.</p> |