发明名称 |
Alphavirus replicons and helper constructs |
摘要 |
The present invention provides a recombinant nucleic acid comprising: a first nucleic acid sequence encoding a 5' alphavirus replication recognition sequence; at least one second nucleic acid sequence encoding an alphavirus nonstructural protein; at least one alphavirus subgenomic promoter; at least one IRES element; at least one heterologous nucleic acid; and a third nucleic acid encoding a 3' alphavirus replication recognition sequence. Further provided are methods of making alphavirus particles comprising a recombinant nucleic acid of this invention and methods of using the compositions of this invention. Also provided is a recombinant helper nucleic acid comprising: a first nucleic acid sequence encoding a 5' alphavirus replication recognition sequence; an alphavirus subgenomic promoter; an IRES element; a second nucleic acid encoding an alphavirus structural protein; and a third nucleic acid encoding a 3' alphavirus replication recognition sequence.
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申请公布号 |
US7442381(B2) |
申请公布日期 |
2008.10.28 |
申请号 |
US20040804331 |
申请日期 |
2004.03.19 |
申请人 |
ALPHAVAX, INC. |
发明人 |
SMITH JONATHAN F.;KAMRUD KURT I.;RAYNER JON O. |
分类号 |
A01N63/00;A61K39/12;C07H21/04;C07K14/16;C07K14/18;C12N7/04;C12N15/86 |
主分类号 |
A01N63/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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