| 摘要 |
Rolling circle replication of a padlock primer is inhibited when it is hybridised to a target nucleic acid that is long or circular. The invention provides methods of addressing this problem including cutting the target nucleic acid to create a fragment, binding a probe having two adjacent sequences complementary to the 3'-end and the 5'-end target sequence of the fragment, circularizing the target nucleic acid and effecting rolling circle replication of the circularised target fragment. Probes and kits for carrying out the method.
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