发明名称 |
FERMENTATION PROCESS FOR CONTINUOUS PLASMID DNA PRODUCTION |
摘要 |
A continuous process is described for the production of microbial plasmid DNA for use in biopharmaceutical and biotechnological applications. The process consists of: first growing microbial cells containing a plasmid at a reduced temperature in a continuous stage; followed by a second plasmid induction continuous culture stage with an increased temperature, with a residence time that allows accumulation of the plasmid product. A hold step at a reduced temperature after fermentation further increases the yield of plasmid product. The method enables production of a large quantity of highly purified plasmid DNA from a small bioreactor over time.
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申请公布号 |
WO2007089873(B1) |
申请公布日期 |
2008.11.20 |
申请号 |
WO2007US02707 |
申请日期 |
2007.01.31 |
申请人 |
NATURE TECHNOLOGY CORPORATION;HODGSON, CLAGUE, P.;CARNES, AARON, E. |
发明人 |
CARNES, AARON, E. |
分类号 |
C12P1/00;C12N15/64;C12P21/04 |
主分类号 |
C12P1/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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