PREPARATION OF STABLE FORMULATION OF LIPID-NUCLEIC ACID COMPLEX EFFECTIVE FOR IN VIVO DELIVERY

摘要

PROBLEM TO BE SOLVED: To provide a lipid-nucleic acid complex that has increased shelf life and shows high transfection activity in vivo after the intravenous injection, and to provide a method for preparing such the complex as this.

SOLUTION: The method generally involves bringing a nucleic acid into contact with an organic polycation to produce a condensed nucleic acid, and then combining the condensed nucleic acid with a lipid comprising an amphipathic cationic lipid to produce the lipid-nucleic acid complex. This complex can be further stabilized by the addition of a hydrophilic polymer attached to a hydrophobic side chain. The complex can also be made specific for special cells, by incorporating a targeting moiety such as a Fab fragment attached to a hydrophobic polymer.

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