| 摘要 |
PROBLEM TO BE SOLVED: To separate each fluorophore from a fluorescence image including different fluorophores in accurate unmixing realized by eliminating positional pixels shifts between different fluorescence images obtained through irradiation with different-wavelength laser light beams. SOLUTION: A laser scanning microscope 1 includes a laser light source 8 capable of changing and emitting the different-wavelength laser light beams, a correction amount determination unit that determines a correction amount for correcting an optical axis shift of the emitted laser light, an optical axis adjusting unit 11 that adjusts an optical axis of the laser light, a scanning unit 14 that performs two-dimensional scanning of the laser light, an objective lens 15 that focuses the scanned laser light to a specimen A and fluorescence emitted from the specimen A, a light detector 29 that detects the focused fluorescence, and a control unit 5 that changes the wavelength of the laser light emitted from the laser light source 8, synchronously with the scanning of the laser light by the scanning unit 14 while controlling the optical axis adjusting unit 11 on the basis of the correction amount determined by the correction amount determination unit. COPYRIGHT: (C)2009,JPO&INPIT
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