发明名称 |
Cultured Cell Sheet, Production Method Thereof, and Application Method Thereof |
摘要 |
The problem to be solved by the present invention is to provide a cultured cell sheet that expresses phenotypes of the chondroid tissue. The problems can be solved by producing the cultured cell sheet in which cells are cultured on a surface of a cell culture support that is coated with a temperature responsive polymer having an upper or lower critical solution temperature ranging from 0° C. to 80° C. in water, including steps of: adjusting the temperature of the culture medium to a temperature below the lower critical solution temperature or above the upper critical solution temperature; bringing the cultured cell sheet in close contact with the carrier; and detaching the cultured cell sheet together with the carrier. |
申请公布号 |
US2008226692(A1) |
申请公布日期 |
2008.09.18 |
申请号 |
US20060885246 |
申请日期 |
2006.02.28 |
申请人 |
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发明人 |
SATO MASATO;SAKAI HIDEAKI |
分类号 |
A61F2/00;A61K35/12;A61P19/00;C12N5/077 |
主分类号 |
A61F2/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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