| 摘要 |
There is provided a real time PCR assay capable of calculating a ratio of the number of mutans streptococci to the number of oral streptococci present in an oral cavity, in a short time. The object can be solved by a method for measuring the number of oral streptococci using the combination of a forward primer comprising a primer oligonucleotide part of at least 15 sequential bases in the base sequence of SEQ ID NO: 1 and a reverse primer comprising a probe oligonucleotide part of at least 15 sequential bases in the base sequence of SEQ ID NO: 2; and a probe for measuring the number of oral streptococci, comprising a nucleotide part consisting of at least 10 sequential bases in the base sequence of SEQ ID NO: 3 or 4. |
