METHOD FOR DETECTING SMALL OLIGONUCLEOTIDES

摘要

A method for detecting small oligonucleotides includes providing a biological isolate containing at least one small oligonucleotide. The biological isolate may be contacted with at least one detection oligonucleotide having a label moiety and at least one bridge oligonucleotide under conditions such that the at least one small oligonucleotide and the at least one detection oligonucleotide are preferentially added to the bridge oligonucleotide to produce at least one labeled small oligonucleotide. At least one ligating reagent may be added to preferentially join the at least one small oligonucleotide and the at least one detection oligonucleotide. The at least one labeled small oligonucleotide may then be detected.