| 摘要 |
<p><P>PROBLEM TO BE SOLVED: To provide a surface plasmon enhanced fluorescence sensor capable of detecting fluorescence with extremely high sensitivity. <P>SOLUTION: The fluorescence sensor is constituted of: a light source 7 emitting excitation light 8 of a predetermined wavelength; a dielectric block 13 formed of a material transmitting the excitation light 8; a metal film 20 formed on a surface 13a of the dielectric block 13; an inflexible film 31, which is made of a hydrophobic material and formed on the metal film 20, with a film thickness within a range of 10 to 100 nm; a sample holding portion 5 holding a sample 1 to bring it into contact with the inflexible film 31; an incident optical system 7 causing the excitation light 8 to enter the interface between the dielectric block 13 and the metal film 20 through the dielectric block 13 such that conditions for total internal reflection are satisfied; and fluorescence detecting means 9 detecting fluorescence emitted by a substance within the sample 1, which is excited by evanescent waves 11 that leak from the interface when the excitation light 8 enters the interface. <P>COPYRIGHT: (C)2008,JPO&INPIT</p> |
