METHOD OF MEASURING LENGTH OF NUCLEIC ACID USING FET BASED BIOSENSOR

摘要

A method for measuring length of a nucleic acid is provided to measure the length of the nucleic acid without destruction or deformation of the nucleic acid using an FET-based bio sensor. A method for measuring length of a nucleic acid comprises the steps of: (a) providing a plurality of test solution having the same ion concentration and containing test nucleic acids with different concentrations to the surface of an insulation layer of an FET based bio sensor; (b) measuring the electric signal change of the FET based bio sensor to obtain data of electric signal change values in accordance with the concentration of the test nucleic acid; (c) obtaining the concentration of the test nucleic acid which has the minimum electric signal change value; (d) obtaining an electric signal change value data in accordance with the nucleic acid concentration using nucleic acids, each of which length is known; and (f) comparing the electric signal change value data in accordance with the nucleic acid concentration with the concentration of the nucleic acid having the minimum electric signal change value using the length-known nucleic acids, wherein the electric signal is at least one of drain current, gate-source voltage and source-drain and the nucleic acid is an oligomer or a PCR product. Further, the nucleic acid is selected from a group consisting of DNA, RNA, PNA, LNA and the mixture.