SITE-SPECIFIC INCORPORATION OF AMINO ACIDS INTO MOLECULES

摘要

The invention provides certain embodiments relating to methods and compositions for incorporating non-natural amino acids into a polypeptid or protein by utilizing a mutant or modified aminoacyl-tRNA synthetase to charge the non-natural amino acid to a the corresponding tRNA. In certain embodiments, the tRNA is also modified such that the complex forms strict Watson-Crick base-pairing with a codon that normally forms wobble base- pairing with unmodified tRNA/aminoacyl-tRNA synthetase pairs.