| 摘要 |
#CMT# #/CMT# Eukaryotic cells transformed by a specific replacement process, in which insert DNA comprising part of a gene is recombined with complement DNA to form a complete recombinant gene in the cells' genome, are new, provided that the insertion site is in a receiver gene containing the complement DNA, the cells are transfected with a vector containing the insert DNA with two flanking sequences homologous to two genomic sequences adjacent to the insertion site. #CMT# : #/CMT# Independent claims are also included for: (1) transgenic animal in which one copy of a gene (G1) of which there are no more than two copies in the animal's genome is inactivated by insertion of a different gene (G2) at a position such that G2 is expressed under the control of regulatory sequences of G1; (2) transgenic animal in which an endogenous gene (G1) is inactivated by insertion of a different gene (G2) at a position such that G2 is expressed under the control of regulatory sequences of G1. #CMT#ACTIVITY : #/CMT# None given. #CMT#MECHANISM OF ACTION : #/CMT# Gene therapy. #CMT#USE : #/CMT# The specific replacement process is useful for gene therapy, for producing transgenic animals and for genetic marking of animals. #CMT#BIOTECHNOLOGY : #/CMT# Preferred Cells: The cells are embryonic stem cells. The insert DNA comprises a coding sequence lacking its promoter. Preferred Animal: G2 is a lacZ gene, a gene coding for an interleukin, interferon or receptor or a disease-associated gene, especially a retinoic acid, beta-3 adrenergic or HIV receptor gene. #CMT#EXAMPLE : #/CMT# Plasmid pGN comprised a lacZ coding sequence flanked by sequences homologous to Hox-3.1 gene sequences. This was used to transfect embryonic mouse cells by electroporation. Several clones expressing beta -galactosidase were isolated. |
