MODIFIED BETA-LACTAMASE AND METHOD FOR ITS PREPARATION

摘要

The invention relates to targeted post translational modification of meta llo-beta-lactamase by truncation and insertion of a dipeptide at the amino t erminal end to reduce amino terminal heterogeneity in a recombinant DNA prod uction system. A protein K-T-E-.DELTA.BL is expressed, and modified by host proteases to E-.DELTA.BL. Appropriate nucleotide molecules, vectors and host s are also described. E-.DELTA.BL is useful in a pharmaceutical composition for treating antibiotic induced adverse effects in the intestine of patients treated with beta-lactam antibiotics.