发明名称 |
Method of Preparing Isolated Cell-Free Skin, Cell-Free Dermal Matrix, Method of Producing the Same and Composite Cultured Skin with The Use of the Cell-Free Dermal Matrix |
摘要 |
An object of the present invention is to provide a method for producing an ADM suitable as a scaffold for a cultured skin, that is, a separation and decellularization method that enables various types of extracellular matrixes such as a basement membrane to be preserved, allows the epidermal layer to be peeled off easily, and does not cause any damage to the dermal matrix. The present invention relates to a skin separation and decellularization method comprising a step of freeze thawing harvested skin and then separating the skin into epidermis and dermis by a treatment with hypertonic saline, and a step of washing the separated dermis.
|
申请公布号 |
US2007269791(A1) |
申请公布日期 |
2007.11.22 |
申请号 |
US20040584630 |
申请日期 |
2004.12.24 |
申请人 |
YOSHISIRO TAKAMI |
发明人 |
TAKAMI YOSHIHIRO;YAMAGUCHI RYO;MATSUDA YASUSHI |
分类号 |
A01N1/00;A61L27/00;A61L27/36;A61L27/60 |
主分类号 |
A01N1/00 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|