METHOD OF DETACHING CELLS FROM THE SURFACE WHICH THE CELLS ADHERE TO

摘要

A method for detaching cells from attached surface with high yield is provided to separate the cells from the surface without the damage of the cells without using an enzyme such as trypsin by introducing chitosan with the characteristic of being depolymerized in a weak acidic range into the inner surface of a cell culture container. A method comprises the steps of: (a) after filling a liquid culture medium in a cell culture container in which a chitosan coating film is formed inside, adhesion-culturing cells on the chitosan coating film; (b) adding an acid such as sulfuric acid, nitric acid and phosphoric acid to the liquid culture medium to adjust the pH of the medium into 4.5-6.0 to depolymerize the chitosan; and (c) isolating the cells from the cell cultured material including the depolymerized chitosan through centrifugation.