摘要 |
Disclosed is a composition of matter of the formula (I) (X<SUP>1</SUP>)<SUB>a</SUB>-(F<SUP>1</SUP>)<SUB>d</SUB>-(X<SUP>2</SUP>)<SUB>b</SUB>-(F<SUP>2</SUP>)<SUB>e</SUB>-(X<SUP>3</SUP>)<SUB>c</SUB> and multimers thereof, in which F1 and F2 are half-life extending moieties, and d and e are each independently 0 or 1, provided that at least one of d and e is 1; X<SUP>1</SUP>, X<SUP>2</SUP>, and X<SUP>3</SUP> are each independently -(L)<SUB>f</SUB>-P-(L)<SUB>g</SUB>-, and f and g are each independently 0 or 1; P is a toxin peptide of no more than about 80 amino acid residues in length, comprising at least two intrapeptide disulfide bonds; L is an optional linker; and a, b, and c are each independently 0 or 1, provided that at least one of a, b and c is 1. Linkage to the half-life extending moiety or moieties increases the in vivo half-life of the toxin peptide, which otherwise would be quickly degraded. A pharmaceutical composition comprises the composition and a pharmaceutically acceptable carrier. Also disclosed are a DNA encoding the inventive composition of matter, an expression vector comprising the DNA, and a host cell comprising the expression vector. Methods of treating an autoimmune disorder, such as, but not limited to, multiple sclerosis, type 1 diabetes, obesity, psoriasis, inflammatory bowel disease, contact-mediated dermatitis, rheumatoid arthritis, psoriatic arthritis, transplant rejection, graft-versus-host disease, and lupus and of preventing or mitigating a relapse of a symptom of multiple sclerosis are also disclosed. |
申请人 |
AMGEN INC. |
发明人 |
SULLIVAN, JOHN K.;MCGIVERN, JOSEPH G.;MIRANDA, LESLIE P.;NGUYEN, HUNG Q.;WALKER, KENNETH W.;HU, SHAW-FEN SYLVIA;GEGG JR., COLIN V.;MCDONOUGH, STEFAN I. |