PROCESS FOR PREPARING AND PURIFYING ALPHA-INTERFERON

摘要

This invention relates to a process for the preparation of recombinant interferon wherein the host organism containing the interferon gene is cultivated, the cells are harvested and disrupted, the cell debris is removed, the interferon is concentrated and subjected to preliminary purification by tandem chromatography, the eluate is adjusted to pH 4.0-4.8 to remove impurities, the interferon is finally purified by chromatography on a cation exchanger column with a volatile buffer as eluant, and is then lyophilised whereby a highly pure, non-immunogenic, homogeneous .alpha.-interferon having anti-viral and immunoregulatory activities is obtained, to the proteins produced by the process and to the use thereof.