CHROMATOGRAPHIC ANALYSIS OF NUCLEIC ACIDS

摘要

A method is disclosed for predicting the retention time of a nucleic acid during chromatographic analysis. Typically, this will be the prediction of the retention time of DNA molecules during DHPLC analysis. The predicted retention time is calculated based on the nucleotide distribution of the nucleic acid, and particular emphasis is placed on the terminal nucleotides. Furthermore, different retention times are predicted depending on whether dideoxynucleotides are present in the nucleic acid. The method can also be used in a software program for the design of multiplex primer extension reactions, enabling high throughput SNP genotyping.