METHOD FOR REGENERATING A PLANT USING INBRED OF CHINESE CABBAGE

摘要

A method for regenerating the plants by using inbred line of Chinese cabbage is provided to increase efficiency of genetic transformation of plants mediated by Agrobacterium, and enhance regeneration rate of plants. The method for regenerating the plants comprises the steps of: (a) sterilizing the inbred seeds of Chinese cabbage; (b) germinating the sterilized seeds for 2-5 days; (c) obtaining the cotyledon or hypocotyl explants from the germinated seeds; (d) culturing the cotyledon or hypocotyl explants in MS medium containing 0.1-5 mg/L of BA(6-benzylaminopurine) and 0.1-0.5 mg/L of NAA(alpha-naphthaleneacetic acid) for 10-30 days to induce shoots; (e) orientating the shoots in MS medium containing NAA to induce root development; and (f) transplanting the root developed young plant to a pot and raising it.