| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide a multiplex PCR method enabling easy primer design and having high amplification efficiency. <P>SOLUTION: The PCR amplification method is a multiplex PCR to use a primer having ≥32 base length, perform the annealing at a temperature lower than the Tm of the primer and perform the long-time extension reaction for 1-10 min. <P>COPYRIGHT: (C)2007,JPO&INPIT |
