METHOD FOR THE ISOLATION OF SPIROPLASMA IN MAMMALS

摘要

The present invention is directed to a method of isolating and culturing spiroplasma by obtaining a tissue sample from a bovine; inoculating embryonated eggs with the tissue sample; mixing fluid from the inoculated egg with culture media; culturing the media mixed with the egg fluid at approximately 32°C for preferably greater than a month to establish a spiroplasma culture.