发明名称 |
Process for production of protein having triple-helical structure |
摘要 |
An objective of the present invention is to provide methods of producing human collagen molecules that are easy to isolate and purify and that have a structure substantially equivalent to that of a natural collagen molecule, wherein host cells that are transduced with a collagen gene synthesize large amounts of human collagen protein derived from a gene introduced into a high exogenous gene expression vector. Another objective of the present invention is to provide collagen molecules produced by the production methods. The present inventors discovered that a large amount of human collagen hardly contaminated with host cell-derived collagen could be produced, by selecting from various mammalian cells a host cell that has low collagen expression and introducing a collagen gene construct into a vector capable of high exogenous gene expression. |
申请公布号 |
AU2006231795(A1) |
申请公布日期 |
2006.10.12 |
申请号 |
AU20060231795 |
申请日期 |
2006.03.31 |
申请人 |
OSAKA PREFECTURAL GOVERNMENT;FUSO PHARMACEUTICAL INDUSTRIES, LTD. |
发明人 |
TETSUO KASE;HIROSHI KISAGI;MIZUKI NISHIHARA;HIROYUKI CHASHI;AKIO KIMURA;HIROSHI KAMIYAMA |
分类号 |
C12N15/09;C07K14/78;C12N5/10;C12P21/02 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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