A METHOD FOR DETERMINATION OF DNA FRAGMENTATION IN ANIMAL SPERM CELL

摘要

The present invention describes a method for the determination of DNA fragmentation in animal sperm cells. Specially it is related to a method to evaluate the integrity of the chromatin/DNA of the sperm cells by means of the sample treatment with a denaturing DNA solution followed, optionally, of a staining; a later treatment with a lysing solution which does not contain protein denaturing detergent, followed optionally by a staining; and an evaluation of the integrity of the chromatin/DNA. The present invention is furthermore related to a kit to evaluate the quality of the animal sperm cells which includes a denaturing DNA solution and a lysing solution which does not contain protein denaturing detergent.