发明名称 |
Topoisomerase linker-mediated amplification methods |
摘要 |
A Vaccinia topoisomerase-adapted linker, which contains an oligonucleotide primer binding site of known sequence, useful for specifically joining the linker to the end of a polynucleotide of unknown sequence and allowing subsequent PCR amplification of the polynucleotide or DNA is provided. Kits containing the invention Vaccinia topoisomerase-adapted linker and one or more linker-specific oligonucleotides for annealing to the linker in PCR are also provided. In addition, the invention provides methods for using the invention linkers in linker-mediated PCR amplification procedures for isolation and optional sequencing of isolated PCR amplification products.
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申请公布号 |
US7078501(B2) |
申请公布日期 |
2006.07.18 |
申请号 |
US20010792875 |
申请日期 |
2001.02.23 |
申请人 |
SOUTH CAROLINA RESEARCH INSTITUTE |
发明人 |
HEYMAN JOHN A.;SZALAI GABOR;FELDER MICHAEL;MADDEN KNUT R. |
分类号 |
C07H21/04;G01N33/53;C12N15/09;C12N15/10;C12P19/34;C12Q1/68;G01N33/566 |
主分类号 |
C07H21/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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