AN IMMUNOPRECIPITATION-BASED METHOD TO PURIFY AND CHARACTERISE BIOLOGICAL MACROMOLECULAR COMPLEXES

摘要

This invention concerns an artificial adapter protein that combines an antibody-binding activity with two affinity tags and its use in isolation of antibody-antigen complexes. Using this adapter protein, complexes can be obtained at good yield and in the high purity necessary for the identification of all biological macromolecules that are associated with the antigen.