<p>It is intended to provide a method of separating the pancreatic islet which comprises: (1) the step of injecting a protection liquid containing a protease inhibitor into the pancreatic duct of the pancreas; (3) the step of decomposing the pancreas into which the protection liquid has been injected; (4) and the step of purifying the decomposed pancreas with the use of a purification solution containing a density gradient agent. It is also intended to provide a protection liquid to be injected into the pancreatic duct; a pancreas preservation liquid for the two layer method; and a pancreas purification solution.</p>
