| 摘要 |
Provided is a probe set having the same level of hybrid stability when a plurality of probes are assigned to an identical nucleic acid to be detected. The probe set is adjusted in the binding strength, for example a Tm value, of each probe in consideration of the nucleotide length of single strand portions located on both sides of a hybrid composed of a nucleic acid to be detected and the probe. If necessary, a probe is assigned to a complementary strand of the nucleic acid to be detected. A probe substrate bound with the probe set is also provided.
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