| 摘要 |
A method for determining the sequence of a polynucleotide, comprising the steps of: i. reacting a target polynucleotide with at least an enzyme that is capable of interacting with and precessing along the polynucleotide, under conditions sufficient to induce enzyme activity; and ii. detecting conformational changes in the enzyme as the enzyme processes along the polynucleotide; for carrying out the method a solid support is used comprising at least one immobilised enzyme capable of interacting with the target polynucleotide and precessing along it the polynucleotide, the enzyme being labelled with one or more detectable labels; and a system for determining the sequence of the polynucleotide comprising such solid support and an apparatus for detecting the label. |
