| 摘要 |
PROBLEM TO BE SOLVED: To provide a magnetic bead and a microbe detection method for detecting a microbe more easily with high sensitivity. SOLUTION: Salmonella 21 is captured by using the magnetic bead (polymyxin B immobilized magnetic bead 31) on which polymyxin B to be specifically reacted with lipo-polysaccaride which is a constituent of a cell cortex of the Salmonella 21 is immobilized. Then, the polymyxin B immobilized magnetic bead 31 is separated by a magnet 25 for magnetic bead separation, and a biotin-labeled antibody 32 is bonded to the Salmonella 21 captured by the separated polymyxin B immobilized magnetic bead 31, and an avidin-luciferase complex 33 is bonded thereto. Then, the Salmonella 21 is detected by an emission reaction using luciferin as a substrate. COPYRIGHT: (C)2006,JPO&NCIPI
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