发明名称 |
METHOD OF SYNTHESIZING cDNA |
摘要 |
<p>A method for synthesizing cDNA possessing a consecutive sequence starting with a nucleotide adjacent to a cap structure of mRNA, which comprises (i) a process for annealing a double-stranded DNA primer and an RNA mixture containing mRNA possessing a cap structure, (ii) a process for preparing a conjugate of an mRNA/cDNA heteroduplex and a double-stranded DNA primer by synthesizing the first-strand cDNA primed with the double-stranded DNA primer using reverse transcriptase, and (iii) a process for circularizing the conjugate of the mRNA/cDNA heteroduplex and the double-stranded DNA primer by joining the 3' and 5' ends of the DNA strand containing cDNA using ligase. This method enables us to efficiently synthesize a full-length cDNA possessing a consecutive sequence starting with a transcription-start-site nucleotide from a small amount of RNA by small processes.</p> |
申请公布号 |
EP1616947(A1) |
申请公布日期 |
2006.01.18 |
申请号 |
EP20040724199 |
申请日期 |
2004.03.29 |
申请人 |
JAPAN AS REPRESENTED BY DIRECTOR GENERAL OF NATIONAL REHABILITATION CENTER FOR PERSONS WITH DISABILITIES;HITACHI INSTRUMENTS SERVICE CO., LTD.;KATO, SEISHI |
发明人 |
KATO, SEISHI;KIMURA, TOMOKO;OHTOKO, KUNIYO |
分类号 |
C12N15/10;C12Q1/68;(IPC1-7):C12N15/09 |
主分类号 |
C12N15/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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