发明名称 |
MUTANT ENDOGLYCOCERAMIDASES WITH ENHANCED SYNTHETIC ACTIVITY |
摘要 |
<p>The present invention relates to a novel endoglycoceramidase whose hydrolytic activity has been substantially reduced or eliminated, such that the enzyme is useful for synthesis of glycolipids from a monosaccharide or oligosaccharide and a ceramide. More specifically, the endoglycoceramidase is a mutant version of a naturally occurring endoglycoceramidase, preferably comprising a mutation within the active site or the nucleophilic site of the enzyme and more preferably comprising a substitution mutation of the Glu residue within the active site or the nucleophilic site. Also disclosed are a method for generating the mutant endoglycoceramidase and a method for enzymatically synthesizing glycolipids using this mutant enzyme.</p> |
申请公布号 |
CA2567581(A1) |
申请公布日期 |
2005.12.15 |
申请号 |
CA20052567581 |
申请日期 |
2005.06.01 |
申请人 |
NEOSE TECHNOLOGIES, INC.;THE UNIVERSITY OF BRITISH COLUMBIA |
发明人 |
DEFREES, SHAWN;JOHNSON, KARL F.;WITHERS, STEPHEN G.;VAUGHAN, MARK |
分类号 |
C12N9/24 |
主分类号 |
C12N9/24 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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