发明名称 |
GENETISCHE KASKADEREGULIERTE EXPRESSION VON KLONIERTEN GENEN |
摘要 |
The present invention relates to the design, preparation and use of cascade regulatory circuits for amplification of gene expression. The genetic circuit is based on a plurality (e.g., two or more) of regulatory genes organized in a hierarchical order of expression in a genetic construct or constructs, which can be established in a cell, e.g., a gram-negative bacteria, by means of autoreplicative vectors or by chromosomal insertion. In one embodiment, the genetic construct(s) can be stabily maintained in the chromosome without selective pressure, and gene expression induced three orders of magnitude therefrom using economical biodegradable benzoate derivatives. |
申请公布号 |
DE60014640(T2) |
申请公布日期 |
2005.10.13 |
申请号 |
DE2000614640T |
申请日期 |
2000.06.22 |
申请人 |
CONSEJO SUPERIOR INVESTIGACIONES CIENTIFICAS, MADRID |
发明人 |
RAMIREZ, CEBOLLA;MARTIN, SOUSA;PRIETO, VICTOR DE LORENZO |
分类号 |
C12N1/21;C12N15/67;C12N15/70 |
主分类号 |
C12N1/21 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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