| 摘要 |
An apparatus for collecting a nucleic acid from a solution is equipped with a container with a solid phase for capturing the nucleic acid by sucking a solution through the solid phase then separating the captured nucleic acid from the solid phase, at controlled discharge and suction rate to provide a nucleic acid recovery rate of not less than 10%, is new. An apparatus for collecting a nucleic acid from a solution is equipped with a container with a solid phase for capturing the nucleic acid by sucking a solution through the solid phase then separating the captured nucleic acid from the solid phase, in which the discharge and suction speeds are controlled by a mechanism so as to elevate the ratio of Nr/Ns to a nucleic acid recovery rate of not less than 10%. Nr/Ns Nr : nucleic acid concentration of the discharged solution; and Ns : nucleic acid of the solution before the suction. Independent claims are also included for: (1) a similar apparatus comprising: (a) a container to hold the sample; (b) a container to retain the collected nucleic acid; (c) a container for capturing a nucleic acid with a space for holding the solution, solid phase for capturing the nucleic acid, opening parts for sucking and discharging such solution, and opening part for adjusting pressure changes in the above space; and (d) a controller to regulate the internal pressure in the space to provide a nucleic acid recovery rate of not less than 10%; (2) a method for collecting a nucleic acid by using the apparatus, optionally with the nucleic acid in the sample at a concentration of 10 2> to 10 6> IU/ml; (3) another apparatus in which a mechanism is installed to calculate Nr and the suction speed, as well as Ns and the discharge speed by basing on information relating to the nucleic acid recover and suction speed or/and discharge speed; (4) a method for collecting a nucleic acid comprising the preparation of a solution with nucleic acid released from the sample, adding a solution to promote binding of the nucleic acid to the solid phase for capture while sucking the solution at not > 1000 Microl/s (except 0) then discharging sucking the solution into such chip then discharging it, removing any components in the solution other than the solid phase-captured nucleic acid continuously, washing the solution, and eluting the captured nucleic acid by sucking and discharging a eluent; (5) yet another apparatus equipped with units to operate sample preparation to release the nucleic acid, capture such nucleic acid, washing the solid phase, and elution of the captured nucleic acid; (6) a method for producing a nucleic acid-containing solution by using the apparatus for recovering such nucleic acid; (7) a method for determining nucleic acid concentration by using the apparatus for collecting such nucleic acid and calculation based on the suction and discharge speeds in relation to the Nr/Ns ratio; and (8) yet another apparatus containing various containers for the sample, recovered nucleic acid, space for controlling pressure and mechanism for adjusting suction and discharge speeds with nucleic acid in the sample at a concentration of 10 2> to 10 6> IU/ml and a definite Nr/Ns ratio. |
