| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide a yeast that has a target protein on the cell cortex and can express the target protein in high efficiency under the industrial environment. <P>SOLUTION: In this practical yeast, from the 5'-terminus in order, a high active promoter selected from the group consisting of yeast SED1 promoter, GAPDH promoter, PGK1 promoter and ADH1 promoter, secretion signal that functions in the yeast cells, the sequence encoding the target protein and a chromosome integration type expression vector that has a sequence encoding the plasma membrane bound area of the GPI anchoring protein are incorporated into the chromosomal DNA. <P>COPYRIGHT: (C)2005,JPO&NCIPI |
