摘要 |
By substituting an amino acid residue at least at one position participating in the efficient formation of (2R,4R)-monatin (100-position, 180- to 183-positions, 243-position and 244-position) in the amino acid sequence of wild type D-aminotransferase represented by SEQ ID NO:2 in Sequence Listing, the D-aminotransferase can be mutated so that it can efficiently produce (2R,4R)-monatin having an elevated sweetness from 4- (indol-3-ylmethyl)-4-hydroxy-2-oxoglutaric acid. |