发明名称 |
SIMPLE ENZYMATIC PROCESS FOR PREPARING CEFAZOLIN |
摘要 |
Preparing cefazolin (A) involves acylating 7-amino-3-[(methyl-1,3,4-thiadiazol-2-yl)thiomethyl]-3-cephem-4-carboxylic acid with tetrazole containing acetic acid in the presence of biocatalyst containing penicillin amidase at pH of 6-8 and at -20 - 40 [deg]C; and precipitating (A) by adding an acid to lower the pH below 3.5 at 0-20 [deg]C. Preparing cefazolin (A) involves acylating 7-amino-3-[(methyl-1,3,4-thiadiazol-2-yl)thiomethyl]-3-cephem-4-carboxylic acid (I) or its salt with tetrazole containing acetic acid of formula (II) in the presence of biocatalyst containing penicillin amidase (EC 3.5.1.11) (100 - 50000 units per liter) at pH of 6 - 8 and at -20 - 40 (preferably 0 - 10)[deg]C; and precipitating (A) by adding an acid to lower the pH below 3.5 at 0 - 20[deg]C. [Image] R : ester or amide of the acid. ACTIVITY : Antibiotic. No biological data is given. MECHANISM OF ACTION : None given. |
申请公布号 |
EP1556500(A1) |
申请公布日期 |
2005.07.27 |
申请号 |
EP20030773688 |
申请日期 |
2003.10.30 |
申请人 |
BIOFERMA MURCIA, S.A. |
发明人 |
GARCIA CARMONA, FRANCISCO;SANCHEZ FERRER, ALVARO |
分类号 |
C07D501/36;C12N9/84;C12P35/04 |
主分类号 |
C07D501/36 |
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