发明名称 |
Method for nucleic acid preparation |
摘要 |
Concentration of oligonucleotides in salt solutions is accomplished by loading a solution of the oligonucleotide dissolved in aqueous sodium chloride or other salt solution onto a reverse-phase poly(styrene-divinylbene) liquid chromatography (LC) column. The column bearing oligonucleotide is then washed with water to low conductivity and eluted with an organic eluent such as ethanol, thus effecting a combination desalting concentration procedure in one step, thus this procedure has utility in the desalting and concentration of oligonucleotides that have been purified and/or treated by anion exchange chromatography. In situ cationic exchange of the associated cation of the oligonucleotide can also be incorporated into the procedure of the new invention.
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申请公布号 |
US2005136458(A1) |
申请公布日期 |
2005.06.23 |
申请号 |
US20040984030 |
申请日期 |
2004.09.14 |
申请人 |
OLIGO THERAPEUTICS INC. |
发明人 |
DALE RODERIC M.;GATTON STEVEN L. |
分类号 |
C07H21/04;C12N15/10;C12Q1/68;(IPC1-7):C12Q1/68 |
主分类号 |
C07H21/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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