| 摘要 |
FIELD: biotechnology, microbiology, broth for bifidus bacteria cultivation. ^ SUBSTANCE: claimed broth for bifidus bacteria cultivation contains as main component pancreatic protein hydrolyzates of casein or forcemeat containing 700-900 mg% of amine nitrogen. Hydrolyzable mixture additionally contains 20-40 g/l of cow or pork liver waste. Broth also contains sodium chloride 4.0-5.0 g; L-cystine hydrochloride 0.10-0.15; microbiological agar 0.75-1.00 g; yeast autolyzate 300.0-400.0 ml; pancreatic casein hydrolyzate containing 700-900 mg% of amine nitrogen, diluted with distilled water up to amine nitrogen content of 140-160 mg%, 300.0-350.0 ml; pancreatic forcemeat hydrolyzate containing 700-900 mg% of amine nitrogen, diluted with distilled water up to amine nitrogen content of 140-160 mg%, 300.0-350.0 ml; 40 % lactose solution 25.0 ml. ^ EFFECT: new nutrient medium completely satisfying to bacterium population requirements. ^ 2 cl, 3 tbl, 3 ex |
