Methods for the synthesis of polynucleotides and combinatorial libraries of polynucleotides

摘要

A method of preparing a polynucleotide having a target sequence from a plurality of oligonucleotides, the method comprising: (a) coupling the oligonucleotides to form a plurality of coupled oligonucleotides, wherein each of the coupled oligonucleotides represents a region of the polynucleotide and shares at least one terminal region of sequence with at least one other coupled oligonucleotide; and (b) assembling the polynucleotide by extension of the coupled oligonucleotides; a library of polynucleotides prepared by the method; a method of preparing a polynucleotide having a target sequence from a plurality of oligonucleotides; and a method of identifying a polynucleotide with a predetermined property.