发明名称 |
Nodavirus-like DNA expression vector and uses therefor |
摘要 |
The present invention describes the production of a nodavirus-based DNA vector that drives abundant expression of foreign genes in a wide variety of cell types. The DNA plasmid is initially transcribed by a host-cell RNA polymerase to produce primary transcripts from which a nodaviral RNA-dependent RNA polymerase (RNA replicase) is translated. These primary transcripts are then amplified by the RNA replicase in an autonomous, cytoplasmic RNA replication. Such a vector is a useful addition to the current arsenal of expression vectors, and well suited to laboratory-scale and larger-scale expression of transcripts and/or proteins in eukaryotic cells.
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申请公布号 |
US6869780(B2) |
申请公布日期 |
2005.03.22 |
申请号 |
US20020230730 |
申请日期 |
2002.11.04 |
申请人 |
THE UAB RESEARCH FOUNDATION |
发明人 |
BALL LAURENCE A.;JOHNSON KYLE L.;JOHNSON KAREN N.;PRICE B. DUANE |
分类号 |
C07K14/08;C12N1/15;C12N1/21;C12N15/40;C12N15/86;(IPC1-7):C12N15/86;C12N15/63;C12N15/64;C12N5/10;C07H21/02 |
主分类号 |
C07K14/08 |
代理机构 |
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