| 摘要 |
The present invention relates to libraries comprising recombinant chimeric proteins, each protein comprising a plurality of distinct consensus amino acid sequences corresponding to amino acid regions that are conserved in a plurality of functionally and/or structurally related proteins. The present invention further relates to methods for preparing the recombinant chimeric proteins and uses thereof, that are less expensive, less labor-intensive and more efficient than procedures used in current available methods. The advantage of the present invention is that shuffling between variable regions that are not necessarily predetermined, while maintaining the consensus backbone, increases the production of active enzymes while producing enzyme variants with high diversity, and better properties.
|
